2020 CPIP-L Case 12: Immunology – Biotin Interference in Immunoassay: A Potential Cause of Diagnostic Error

2020 CPIP-L Case 12: Immunology – Biotin Interference in Immunoassay: A Potential Cause of Diagnostic Error is organized by College of American Pathologists (CAP).,Launch Date: December 21, 2020,Expiration Date: December 20, 2023 12:00 AM (ET),,Description:,Biotin (vitamin B7) is widely used in amounts far above the recommended amount as a supplement to improve skin and hair appearance, and as a treatment for certain disorders. Because the attachment of biotin to streptavidin is often used as part of the detection system in immunoassays, large amounts of biotin in serum samples may result in erroneous results that can vary by manufacturer and assay. This activity will cover patterns of biotin interference and steps laboratories can take to minimize the effects of this interference.,,Objectives:,As a result of participating in this activity, you should be able to:,• Describe the usage, dosage, and pharmacokinetics of biotin.,• Recognize the impact of biotin on immunoassays.,• Explain the consequences of biotin interference from a clinical standpoint.,• Identify the possible approaches to prevent consequences of biotin interference.